薛 瑾^1,2，陈灏文^1,2，利晓滢^1,2，李伟略^1,2，刘丽萍^1,2，曹亚兰^1,2*

（1.广东一方制药有限公司，广东佛山 528000；

2.广东省中药配方颗粒企业重点实验室，广东佛山 528000）

摘要：通过单因素试验考察超声功率、料液比、乙醇体积分数、提取时间对芝麻素得率的影响，建立提取条件与芝麻素得率之间的回归方程，采用Design Expert软件优化得到最佳提取工艺条件。超声辅助提取黑芝麻粕中芝麻素的最佳条件为提取时间1.9 h、料液比1∶25 g/mL、乙醇体积分数83%、超声功率400 W，在此条件下芝麻素得率为66.70 mg/100 g，与预测值基本符合。抗氧化活性试验表明，芝麻素对DPPH自由基和ABTS⁺自由基具有较强的清除作用。研究结果可为黑芝麻粕的功能性成分提取及下游产业研发提供参考。

关键词：超声提取；黑芝麻粕；芝麻素；响应面法

中图分类号：TS229   文献标识码：A   文章编号：1674-506X（2023）04-0051-0007

Optimization of Ultrasound-assisted Extraction Process of Sesamin from Black Sesame Meal by Response Surface Methodology

XUE Jin, CHEN Haowen, LI Xiaoying, LI Weilue, LIU Liping, CAO Yalan^*

（1.Guangdong Yifang Pharmaceutical Co., Ltd., Foshan Guangdong 528000, China;

2.Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,

Foshan Guangdong 528000, China）

Abstract：The effects of ultrasonic power, solid-liquid ratio, ethanol volume fraction and extraction time on the yield of sesamin were studied by single factor experiment. The regression equation between factors and the yield of sesamin was established, and the best formula was optimized by Design Expert statistical software. The optimal extraction conditions of sesamin were obtained as follows: extraction time 1.9 h, solid-liquid ratio 1:25 g/mL, ethanol volume fraction 83% and ultrasonic power 400 W. Under these conditions, the yield of sesamin was 66.70 mg/100 g, which is close to the theoretical values. The antioxidant activity results showed that sesamin from black sesame meal exhibited a strong scavenging effect on DPPH free radicals and ABTS⁺ free radicals. The research can provide reference for the extraction of functional components of black sesame meal and the research and development of downstream industries.

Keywords：ultrasound extraction; black sesame meal; sesamin; response surface methodology

doi：10.3969/j.issn.1674-506X.2023.04-008

收稿日期：2022-09-02

基金项目：国家工业和信息化部2019年产业技术基础公共服务平台项目（2019-00902-1-2）

作者简介：薛瑾（1995-），女，硕士。研究方向：保健食品及功能性食品研发。

*通信作者：曹亚兰（1987-），女，硕士，工程师。研究方向：保健食品及功能性食品研发。

引用格式：薛瑾,陈灏文,利晓滢,等.响应面优化超声提取黑芝麻粕中芝麻素的工艺[J].食品与发酵科技,2023,59(4):51-57.